Cecchini NM et al. Plant J. 2020
Contact : +54 351 5353855
Cecchini NM et al. Plant J. 2020
Guido ME et al. 2020. Cell Mol Neurobiol
Prucca CG et al. Biochem J. 2020
Andrea G Albarracín Orio et al. Commun Biol. 2020
incorporation Dr.. ANC and Fidelio
Zorgniotti et al. Neurobiol Dis. 2021
Lardone et al. Sci. Rep. 2020
Socas LBP. BBA-Biomem. 2020
Zulueta Díaz AND BBA-Biomem. 2020
Romero J. Arch Biochem Biophys. 2020
Smith et al. Plant Physiol Biochem. 2020
Cejas et al. Biol Chem. 2019
Rodríguez-Berdini et al. J Biol Chem. 2020
Puster et al. Cells 2020
Colque CA et al. Antimicrob Agents Chemother. 2020
incorporation Dr.. Argaraña ANC
Vilcaes et al. Int J Mol Sci. 2020
Cardozo Gizzi et al. Nat Protoc. 2020
Simultaneous observation of 3D chromatin organization and transcription at the single-cell level and with high spatial resolution may hold the key to unveiling the mechanisms regulating embryonic development, cell differentiation and even disease. We recently developed Hi-M, a technology that enables the sequential labeling, 3D imaging and localization of multiple genomic DNA loci, together with RNA expression, in single cells within whole, intact Drosophila embryos. Importantly, Hi-M enables simultaneous detection of RNA expression and chromosome organization without requiring sample unmounting and primary probe rehybridization. Here, we provide a step-by-step protocol describing the design of probes, the preparation of samples, the stable immobilization of embryos in microfluidic chambers, and the complete procedure for image acquisition. The combined RNA/DNA fluorescence in situ hybridization procedure takes 4–5 d, including embryo collection. In addition, we describe image analysis software to segment nuclei, detect genomic spots, correct for drift and produce Hi-M matrices. A typical Hi-M experiment takes 1–2 d to complete all rounds of labeling and imaging and 4 additional days for image analysis. This technology can be easily expanded to investigate cell differentiation in cultured cells or organization of chromatin within complex tissues.
Authors: Cardozo Gizzi AM, Espinola SM, Gurgo J, Houbron C, JB plug, DI Cats, Nollmann M.
Article: Direct and simultaneous observation of transcription and chromosome architecture in single cells with Hi-M. Cardozo Gizzi AM, Espinola SM, Gurgo J, Houbron C, JB plug, DI Cats, Nollmann M. Nat Protoc. 2020 Jan 22. doi: 10.1038/s41596-019-0269-9
Ruggiero et al. Immunol Cell Biol. 2020
08/05/2020-12:30 hs. Defense in remote mode
CIQUIBIC among the selected projects
Cardozo Gizzi A. et al. J Mol Biol. 2020
Chumpen S. et al. Biosci Rep. 2020
15/04/2020-10:00 hs. Defense in remote mode.
Malcolm M. et al. Front Cell Neurosci. 2019
Zulueta Y. et al. Colloids Surf B Biointerfaces. 2020
Curtins JA et al. Biochem J. 2019
Lic. Antonella Colque will make a stay in Denmark
Crossio M. et al. Biomolecules. 2019
Huge loss to our institution
A Mangiarotti. et al. BBA-Biomembr. 2019
Monday 16December 2019 to12:30hs– Integrative Building Auditorium. FCQ.
Curtino & Any, Biochem J, 2019.
Researchers promoted in CONICET
It adds to the National Academy of Sciences
Fellow of CIQUIBIC honored for her work presented at SAIB 2019
Rios M. et al. Front Cell Neurosci. 2019
Moyano. et al. Burns. 2019
By Dr. Horacio de la Iglesia (University of Washington)
Marian ME & Fidelio GD. Front Plant Sci. 2019.
Thursday 19of Septemberfrom 2019to11:00hs– Integrative Building Auditorium. FCQ.
Bertoldi ML et al. Front Cell Neurosci. 2019
Monday 9 of September from 2019 to 12:00hs– Integrative Building Auditorium. Fcq.
Quiroga & Valdez Meth. Mol. Biol. 2019
Garay-Novillo JN et al. Front Microbiol. 2019
By Dr. Soler Illia (INS UNSAM)
Racca et al. Front. Oncol. 2019
Zlocowski et al. Sci. Rep. 2019
Cecchini et al. bio-protocol 2019
By Dr. Kropff (IIBBA-CONICET)
Incorporation Drs ACAL. Daniotti and Guido
Benedetto, Front Cell Neurosci. 2019
Dr. Kierbel (IIBIO-INTECH-CONICET)
Luján et al. EnvirMolMut 2019
Szymanowski et al. BBA 2019