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Experimental Guillain-Barre syndrome induced by immunization with gangliosides: Keyhole limpet hemocyanin is required for disease triggering

Funes SC et al, Biochim. Biophys. Acta, 2017

An experimental model of Guillain-Barré Syndrome has been established in recent years. Rabbits develop disease upon immunization with a single dose of an emulsion containing bovine brain gangliosides, KLH and complete Freund’s adjuvant. Within a period of four to ten weeks after immunization, they began to produce anti-ganglioside IgG-antibodies first, and to show clinical signs of neuropathy afterwards. In addition to gangliosides, KLH is a requirement for antibody production and disease triggering. Although KLH is commonly used as an immunological carrier protein, an anti-KLH-specific immune response was necessary for induction of both events. KLH is a glycoprotein carrying most of the immunogenicity in its glycan moiety. Between 20% to 80% of anti-ganglioside IgG-antibodies present in sick rabbit sera cross-reacted with KLH, indicating that both immune responses are related. The terminal Gal-ß(1,3)-GalNAc glycan (present in gangliosides and KLH) is proposed as “key” antigenic determinant involved in inducing the anti-ganglioside immune response. These results are discussed in the context of the “binding site drift” hypothesis.

Autores: Funes SC, Chiari ME, Comín R, Irazoqui FJ, Nores GA.

Artículo: Funes SC et al, Biochim Biophys Acta. 2017 Feb 7. pii: S0925-4439(17)30048-0. doi: 10.1016/j.bbadis.2017.02.007.