Assistant Researcher CONICET / Assistant Professor UNC
Director: Marta E. Hallak
Phone: +54 351 5353855 x 3442
Study of Post-translational Arginylation of Proteins in the Nervous System
The post-translational modifications of proteins play a preponderant role in the modulation of the various biological processes that take place within cell. Among the different modifications known till now, the post-translational arginylation of proteins is gaining greater relevance given the characterization of distinct physiological processes in which it is involved. This modification is carried out by the enzyme Arg-tRNA-protein transferase (Ate-1), which transfers an Arg residue from Arginyl-tRNA to different proteins and polypeptides that preferentially bear an acidic-N-terminal residue. This function of Ate-1 is associated with the subsequent degradation of differing proteins by the ubiquitin-dependent proteasomal pathway. In addition, growing evidences obtained from the identification and characterization of substrates that would not follow this route of degradation, as well as the different studies performed with Ate-1 deficient mice, suggest that Ate-1 has significant relevance in cellular physiology, participating in processes such as cardiovascular development, angiogenesis and gametogenesis, among others. Our investigations are aimed to determine the physiological relevance of Ate-1 in the distinct cellular components of the nervous system, emphasizing its participation in neurodegenerative processes.
- Shahmoradian S.H., Galiano M.R., Chen S., Rasband M.N., Wu C., Mobley W.C., Chiu In. Preparation of Primary Neurons for Visualizing Neurites in a Frozen-Hydrated State Using Cryo-Electron Microscopy and Tomography. Journal of Visualized Experiments 2014 doi:10.3791/50783.
- Huang W., Zhu P.J., Zhang S., Zhou H., Stoica L., Galiano M., Krnjević K., Roman G., Costa-Mattioli M. mTORC2 controls actin polymerization required for consolidation of long-term memory. Nature Neurosci. 2013; 16(4):441-448.
- Galiano MR, Jha S, Ho T, Zhang C, Ogawa Y, Chang K, Stankewich M, Mohler P, Rasband MN. A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly. Cell 2012; 149(5):1125-1139.
- Decca MB, Carpio MA, Forest C, Galiano MR, Job D, Andrieux A and Hallak ME. Post-Translational Arginylation of Calreticulin: A new isospecies of calreticulin component of Stress Granules. J Biol Chem. 2007; 282:8237-8245.
- Galiano M.R., A. Andrieux, Deloulme J.C., Forest C., Schweitzer A., Job D. and Hallak M.E. Myelin basic protein functions as a microtubule stabilizing protein in differentiated oligodendrocytes J. Neurosc. Res.2006; 84:534-541.