DURAND, Sandra

CONICET Research Associate / Assistant Professor UNC
Phone: +54 351 5353855 internal 3442
E-mail: edithsandradurand@gmail.com

Research Topic

Regulation of the myelin basic protein function by calmoduline and post-tranlational modifications

The myelin basic protein (MBP) is essential for the formation of the myelin sheath in the central nervous system. MBP’s interaction with different cellular components is regulated by calmodulin (CaM). In addition, this interaction is influenced by various post-translational modifications (MPT) undergone by MBP. One of the MPT is the deimination or citrullination which correlates with the early development, demyelination and severity of disease in the case of multiple sclerosis (MS).

In order to contribute to the knowledge of the MBP regulation by CaM we study the interaction between the different isoforms of classical MBP family and CaM and also how the deimination/citrullination of MBP modified or not this interaction.

Our findings allow us to affirm that the biological activity of each isoform of MBP is differentially regulated by CaM and/or MPT. Therefore, the study of this regulation may be important in understanding the role of these isoforms in the differentiation of oligodendrocytes, the myelination in normal and pathological conditions as in MS, etc., and as the deimination influences those events.

Publications

• Altered mitochondrial gene expression in human gestational trophoblastic diseases. Durand S., Dumur C., Flury A., Abadie P., Patrito L., Podhajcer O. and Genti-Raimondi S. Placenta 22: 220-226, 2001.
• Identification of multiple differentially expressed messenger RNAs in normal and pathological trophoblast. Durand S., Abadie P., Angeletti S. and Genti-Raimondi S. Placenta 24: 209-218, 2003.
• GTT1 / StarD7, a novel phosphatidylcholine transfer protein-like highly expressed in gestational trophoblastic tumour: cloning and characterization. Durand S., Angeletti S. and Genti-Raimondi S. Placenta 25: 37-44, 2004.
• The arginylation-dependent association of calreticulin with stress granules is regulated by calcium. Carpio M.A., López Sambrooks C., Durand E.S. and Hallak M.E. Biochemical Journal 429: 63-72, 2010.
• Calreticulin-dimerization induced by post-translational arginylation is critical for stress granules scaffolding. Carpio M.A., Decca M.B., Lopez Sambrooks C., Durand E.S., Montich G.G. and Hallak M.E. The International Journal of Biochemistry and Cell Biology 45: 1223-1235, 2013.

Collaborators

• Christophe Bosc there Annie Andrieux – Research Centre Inserm U 836-UJF-CEA-CHU, Grenoble. Neuroscience Institute, Université Joseph Fourier. Faculty of Medicine, Field of Thanks, F-38700 La Tronche, France

Brief CV

Academic Formation

• Elementary and Middle School: Manuel Belgrano School of Villa Huidobro, General Roca Department, Cordoba Province. Year of graduation: 1980.
• High School: Private Institute Incorporated to formal education: “The Sacred Hearts”” of Villa Huidobro, General Roca Department, Cordoba Province. Year of graduation: 1985. Degree: Bachelor in Teaching Guidance.
• University: National University of Córdoba, College of Chemical Sciences. Graduation date: 10/08/1991. Degree: Biochemistry.
• Oncohematology Laboratory Training: National University of Córdoba, Faculty of Medicine, National Hospital of Clinical, Oncohematology Department, from 01/06/1991 to 06/11/1996.
• PhD: National University of Córdoba, College of Chemical Sciences, Clinical Biochemistry Department. Registered in the PhD Career on 23 May, 1997 and accepted on 13 August, 1997. Graduation date: 11/08/2003. Degree: PhD in Chemistry. PhD title: “Identification of genes differentially expressed in normal and pathological trophoblast. Characterization of a novel transcript encoding a protein with lipid binding domain”.
• PosDoc: National University of Córdoba, College of Chemical Sciences, Biological Chemistry Department, from 01/04/2004 to 30/06/2005. PosDoc title: “Control study of the phospholipid biosynthesis rate in tumor cells activated by c-Fos through a nongenomic mechanism with therapeutic purposes”.
• Assistant Research of the Career of Scientific and Technological Research (CICYT-CONICET) from 01/07/2005 to 31/05/2013. Discipline: Biochemistry.
• Associate Research of the Career of Scientific and Technological Research (CICYT-CONICET) since 01/06/2013 to date.
• Positions of Auxiliary Professor at the Clinical Biochemistry Department, College of Chemical Sciences, National University of Córdoba, from 01/12/1992 to 31/03/2004.
• Positions of Auxiliary Professor at the Biological Chemistry Department, College of Chemical Sciences, National University of Córdoba, from 01/07/2004 to 31/03/2008.
• Position of Assistant Professor at the Biological Chemistry Department, College of Chemical Sciences, National University of Cordoba, since 01/04/2008 to date.
• Teacher-Researcher from Incentive Program of the Ministry of Culture and Education – University Policies Secretariat; category V: 1994-1999, category IV: 2000-2004 and category III since 2005 to date.
• Referee of the Argentine Accreditation Organism (OAA). Date appointed: 31/05/2011; in activity since then to date.

Research Background

Publications

• Chronic Myelogenous Leukemia: relationship between the bcr-abl molecular pattern and cellular phenotype of blast crisis. Durand S., Rodriguez C., Bocco J. and Nunez B. Presence Biochemistry Biochemists Association of Córdoba No. 178, volume 15: 11-18, March 1997.
• Clinical and molecular diagnosis of myotonic dystrophy. Durand S., Slavin D., Molina de Levstein I. y Bocco J.L. Alberto J Annals of the Foundation. Roemmers, Volume XII: 49-56, 1999.
• Identification of Trypanosoma cruzi zymodemes by kinetoplast DNA probes. Montamat E. E., Durand S., Bocco J.L., De Luca d’Oro G.M. and Blanco A. Journal of Eucaryotic Microbiology 46: 155-159, 1999.
• Molecular mechanisms implicated in galectin-1-induced apoptosis: activation of the AP-1 transcription factor and downregulation of Bcl-2. Rabinovich G.A., Alonso C.R., Sotomayor C.E., Durand S., Bocco J.L. and Riera C.M. Cell Death and Differentiation 7: 747-753, 2000.
• Altered mitochondrial gene expression in human gestational trophoblastic diseases. Durand S., Dumur C., Flury A., Abadie P., Patrito L., Podhajcer O. and Genti-Raimondi S. Placenta 22: 220-226, 2001.
• A new death domain associated with gestational trophoblastic diseases induces apoptosis in distinct cell types. Atrophy C.l., Almenara J.A., Durand S., Flury A., Koritschoner N.P. and Patrito L.C. International Journal of Oncology 19: 1161-1167, 2001.
• Identification of multiple differentially expressed messenger RNAs in normal and pathological trophoblast. Durand S., Abadie P., Angeletti S. and Genti-Raimondi S. Placenta 24: 209-218, 2003.
• GTT1 / StarD7, a novel phosphatidylcholine transfer protein-like highly expressed in gestational trophoblastic tumour: cloning and characterization. Durand S., Angeletti S. and Genti-Raimondi S. Placenta 25: 37-44, 2004.
• Evaluation of stromal metalloproteinases and vascular endothelial growth factors in a spontaneous metastasis model. Donadio A.C., Durand S., Remedi M.M., Fred S., Ceschin D. G., People-Raimondi S. and Chiabrando G.A. Experimental and Molecular Pathology 79: 259-264, 2005.
• The arginylation-dependent association of calreticulin with stress granules is regulated by calcium. Carpio M.A., López Sambrooks C., Durand E.S. and Hallak M.E. Biochemical Journal 429: 63-72, 2010.
• Galectin-1 confers immune privilege to human trophoblast: implications in recurrent fetal loss. Ramhorst R.E., Giribaldi L., Fraccaroli L., Toscano M.A., Stupirski J. C., Romero M.D., Durand E.S., Rubinstein N., Blaschitz A., Sedlmayr P, People-Raimondi St., Fainboim L. and Rabinovich G.A. Glycobiology 22: 1374-1386, 2012.
• Calreticulin-dimerization induced by post-translational arginylation is critical for stress granules scaffolding. Carpio M.A., Decca M.B., Lopez Sambrooks C., Durand E.S., Montich G.G. and Hallak M.E. The International Journal of Biochemistry and Cell Biology 45: 1223-1235, 2013.

Teaching Background

I did my degree in Biochemistry at the Faculty of Chemistry, National University of Córdoba (FCQ-UNC) (1986-1991). I worked in the laboratory of the Oncohematology Department, National Hospital of Clinical, Faculty of Medicine, UNC (1991-1996). Since 1992 I teach at the FCQ-UNC. I did my PhD at the Clinical Biochemistry Department, FCQ-UNC (1997-2003). I did my postdoc at the Biological Chemistry Department, FCQ-UNC (2004-2005). I started the Career of Scientific Research of the National Council of Scientific and Technical Research (CICYT-CONICET) in 2005, being actually Associate Research. In 2011 I started working for the Argentine Accreditation Organism (OAA). Throughout my professional (Biochemistry) and scientific (PhD in Chemistry) career I have specialized in molecular biology techniques for diagnosis and research respectively. In recent years I have added to my expertise cell biology techniques in order to analyze the effect of post-translational modifications in cell function.

Institutional Management Background

• Member of the Commission on Practical Work by the Biological Chemistry Department, College of Chemical Sciences, National University of Córdoba, during the years 2004, 2005 and 2006.
• Tutor of the Tutoring Institutional Program by the Biological Chemistry Department, College of Chemical Sciences, National University of Córdoba, from June 16, 2006.
• Member of the Departmental Council for the Biological Chemistry Department, College of Chemical Sciences, National University of Córdoba, from 14 October 2010 until 13 October 2012.
• Delegate of the Biological Chemistry Department for the organization of the 6th Conference of Graduate Faculty of Chemistry, National University of Córdoba, held during 5 and 6 December 2013.