Associate Researcher CONICET / Assistant Professor, School of Chemistry, UNC
Phone:+54 351 5353588 int. 3413
E-mail: ecarrizo@fcq.unc.edu.ar
Research Topic
Molecular glycobiology
Our studies are focused on the analysis of proteins involved in the recognition and/or metabolism of carbohydrates, through a structural-functional approach.
In particular, we are interested in the following topics:
- Molecular basis of glycogen metabolism and its dysfunctions: the synthesis and degradation of glycogen are relatively simple biochemical processes whose regulation is quite complex. Several enzymes and regulatory proteins are involved in these processes and defects in some of them can make the stored glycogen to be abnormal either in quantity or in structure. These disorders can have serious consequences and in some cases, they may even cause death. Our goal is to study at molecular level of some of these proteins to understand the structural basis of their mechanisms of action and their malfunction in the pathologies.
- Molecular study of lectins with potential clinical or biotechnological relevance: Carbohydrates have several functions, such as the structure modulation or modification of the molecules they are bound to or the participation in physiological or pathological processes when they are recognized by carbohydrate binding proteins called lectins. Given the huge variety of glycans that can be found in different organisms, it is very interesting to identify the mechanism that makes a lectin to recognize a specific type of glycan. Our goal is to determine the molecular details of this recognition to develop greater understanding of the process in which it is involved.
Fellows
Selected Publications
- Crystallization and preliminary X-ray studies of the common edible mushroom (Agaricus bisporus) lectin. Mary E. Carrizo, Fernando J. Irazoqui, Ricardo L. Lardone, Gustavo A. Nores, Juan A. Curtino, Stefano Capaldi, Massimiliano Perduca & Hugo L. Monaco (2004) Acta Crystallogr. D60, 718-720.(doi:10.1107/S0907444904001969 )
- The antineoplastic lectin of the common edible mushroom (Agaricus bisporus) has two binding sites, each specific for a different configuration at a single epimeric hydroxyl. Mary E. Carrizo, Stefano Capaldi, Massimiliano Perduca, Fernando J. Irazoqui, Gustavo A. Nores & Hugo L. Monaco (2005) J. Biol. Chem. 280, 10614-10623. (doi:10.1074/jbc.M411989200)
- Structure of a lectin with antitumoral properties in king bolete (Boletus edulis) mushrooms. Michele Bovi, Mary E. Carrizo, Stefano Capaldi, Massimiliano Perduca, Laurent R. Chiarelli, Monica Galliano & Hugo L. Monaco (2011) Glycobiology 21,1000-1009. (doi: 10.1093/glycob / cwr012)
- Mechanisms of monomeric and dimeric glycogenin autoglucosylation. Federico M. Issoglio, Mary E. Carrizo, Jorge M. Romero & Juan A. Curtino (2012) J. Biol. Chem. 287, 1955-1961. (doi:10.1074/jbc.M111.287813)
- Structural and biochemical insight into glycogenin inactivation by the glycogenosis-causing T82M mutation. Mary E. Carrizo*, Jorge M. Romero, Federico M. Issoglio & Juan A. Curtino (2012) FEBS Lett. 586, 254-257. * Author Responsible
(See more publications-CONICET)
Brief CV
Academic Formation
- 1994 – Bachelor of Chemistry (Degree in Chemistry). University of Córdoba
- 2000 – PhD in Chemical Sciences. University of Córdoba.
Research Background
- 1995 – 2001: Research Fellow. Advisor: Prof. Juan A. Curtino. CIQUIBIC-CONICET and Department of Biological Chemistry, College of Chemical Sciences, University of Córdoba.
- 2001 – 2004: Postdoctoral training. Advisor: Prof. Hugo L. Monaco. Biocrystallography Laboratory, Department of Science and Technology, University of Verona, Italy.
- 2005 – present: Member of the Research Career, National Research Council of Argentina (CONICET). CIQUIBIC-CONICET and Department of Biological Chemistry, College of Chemical Sciences, University of Córdoba”
Teaching Background
- 1993 – 1994: Teaching fellow (Teaching assistant). Department of Biological Chemistry, School of Chemistry, University of Córdoba.
- 1994-2001: Instructor (Teaching Assistant). Department of Biological Chemistry, School of Chemistry, University of Córdoba.
- 2005 – present: Assistant Professor. Department of Biological Chemistry, School of Chemistry, University of Córdoba.