Assistant Researcher CONICET / Assistant professor (DS) UNC
Phone:+54-351-5353855 internal 3441
E-mail: mcarpio@fcq.unc.edu.ar
Research Topic
Apoptosis regulation mediated by calcium and stress
Apoptosis is a central biological process, necessary for the development and maintenance of the homeostasis of any tissue. Non regulation of apoptosis results in the excessive disappearance of cells, as is the case, for example, in myocardial infarction and in most cardiovascular diseases (CVDs) but it can also induce pathological cell survival as observed in the development of cancer. On the contrary, the inhibition of apoptosis is the one that plays an essential role in the tumor process and the search for effective treatments is today one of the great challenges of science and medicine. An extensive family of Bcl-2 proteins, comprising a network of “guardian” and “executioner” proteins, regulate cell life and death during most of these events. The suppression, alteration in the expression and functionality of these proteins contributes to the pathogenesis and progression of human cancers and ECVs, thus providing new targets for the discovery of drugs that are currently being studied in human clinical trials.
In addition to this family of proteins specialized in apoptotic control, proteins such as calreticulin, a ER protein involved in the regulation and storage of intracellular Ca2+, which has been shown to be involved in tumorigenesis, facilitating proliferation, migration and adhesion. In view of current trends in research, it can be stated that the impossibility of avoiding spontaneous apoptosis in CVDs and, in the opposite direction, the failure to induce selective apoptosis in cancer cells and tumors. In the present project, CRT-mediated apoptosis and proteins of the BCL-2 family will be studied in the translational control of anti- / pro-apoptotic proteins via stress granules and calcium transfer through membranes associated with mitochondria (MAMs) and the participation of these mechanisms in cardiac pathology and possible translational implications in different types of cancer.
Selected Publications
- Mark A. Carpio, Michael Michaud, Wenping Zhou, Jill K. Fisher, Loren D. Walensky, and Samuel G. Katz. Role of BOK at the intersection of endoplasmic reticulum stress and apoptosis regulation. Proc Natl Acad Sci U S A (PNAS). 2016 Feb 2; 113 (5):E494-5
- Mark A. Carpio, Michael Michaud, Wenping Zhou, Jill K. Fisher, Loren D. Walensky and Samuel G. Katz. BCL-2 family member BOK promotes apoptosis in response to endoplasmic reticulum stress. Proc Natl Acad Sci U S A (PNAS). 2015 Jun 9; 112 (23): 7201–7206.
- Mark A. Carpio, Maria B. Decca, Cecilia Lopez Sambrooks, Edith S. Durand, Guillermo Montich and Marta E. Hallak. Calreticulin-dimerization induced by post-translational arginylation is critical for stress granules scaffolding. The International Journal of Biochemistry and Cellular Biology, (IJBCB). Volume 45, Issue 7, July 2013, Pages 1223–1235.
- Cecilia Lopez Sambrooks, Mark A. Carpio, and Marta E. Hallak. Arginylated calreticulin at the plasma membrane increases the susceptibility of cells to apoptosis. J. Biol. Chem. (JBC), Vol 287, Number 26, 22043-22054, June 22, 2012.
- Mark A. Carpio, Cecilia Lopez Sambrooks, Edith S. Durand and Marta E. Hallak. The arginylation-dependent association of calreticulin to stress granules is regulated by calcium. Biochem. J., Volume 429, part 1, 63-72, 1 July 2010.
Collaborators
Brief CV
Academic Formation
- Postgraduate University Degree / PhD: Doctor of Chemical Sciences, awarded by the Faculty of Cs. Chemistry, National University of Córdoba, 2011.
- University degree: Bachelor in Chemistry, awarded by the Faculty of Cs. Chemicals, National University of Córdoba, dated April 2006.
Research Background
- ASSOCIATED SCIENTIFIC RESEARCHER, Department of Pathology, School of Medicine, Yale University, USA. February 2016 – July 2017, under the direction of MD, PhD Samuel Katz. “Role of BOK at the intersection of endoplasmic reticulum stress and apoptosis regulation”
- POSTDOCTORATE ASSOCIATE to the Department of Pathology, School of Medicine, Yale University, USA. January 2013 – January 2016, under the direction of MD, PhD Samuel Katz. “Role of BOK at the intersection of endoplasmic reticulum stress and apoptosis regulation”
- POSTDOCTORAL SCHOLARSHIP CONICET. April 2011 – December 2012. directed by Dr. Marta Hallak. Dept. of Biological Chemistry, Faculty of Chemical Sciences, National University of Córdoba. cordoba Argentina.
- CONICET DOCTORAL INTERNAL SCHOLARSHIP (Type I and II). April 2006 – March 2011. directed by Dr. Marta Hallak. Topic: “Post-translational modifications of proteins. Biochemical, molecular and functional studies of arginylated proteins “Dept. of Biological Chemistry, Faculty of Chemical Sciences, National University of Córdoba.
- PROFESSIONAL PRACTICANATO OF LICENCIATURE IN CHEMISTRY in the research group led by Dr. Marta Hallak. February 2004 – December 2004. Dept. of Biological Chemistry, Faculty of Chemical Sciences, National University of Córdoba.
Teaching Background
- Assistant Professor (DS), Department of Biological Chemistry Ranwel Caputto. Appointed to the preparation, dictation and evaluation of practical classes of the subject “Laboratory IV”.
- Assistant Professor (FROM), Department of Biological Chemistry Ranwel Caputto. Appointed to the preparation, dictation and evaluation of practical classes of the subject “Laboratory IV”. August 2017- September 2017: Assistant Professor A (FROM), Interim Position Dedication: Exclusive. Department of Biological Chemistry Ranwel Caputto. Appointed to the preparation, dictation and evaluation of practical classes of the subjects “Cellular and Molecular Biology” and “Laboratory IV”.