Senior Researcher CONICET / Full Professor UNC
Phone: +54 351 5353855 ext 3465
E-mail: gerardo.fidelio@gmail.com
Research Topic
Biophysical studies of membrane active proteins (phospholipases) and bioactive peptides with organized lipid interfaces.
My research interest is in the field of protein and membrane biophysics. The main research topics are protein stability, protein-ligand stability, protein-protein interaction, protein-lipid interaction (including enzymes such as phospholipase A2) and the interaction of amphiphilic peptides with organized phospholipids.
Current research projects
General:
Biophysical studies of protein-protein and lipid-protein interaction.
Specific:
- a) Sequence, structure and catalytic properties of phospholipases A2.
- b) Interaction of amphiphilic peptides with organized phospholipids. (Collaboration with Ambroggio, Ernesto)
Fellows
Selected Publications
- In silico and in vitro characterization of phospholipase A2 isoforms from soybean (Glycine max). Marian ME, Villarreal MA, Cheung F, EPM Leiva, Madoery RR and Fidelio GD. Biochimie Dec 2012, 94(12):2608-19.
- Cloning and functional expression of secreted phospholipases A2 from Bothrops diporus (Yarará Chica) Yunes Quartino PJ, Barra JL and Fidelio GD. Biochem. Biophys. Res. Comm. 2012 Oct 19; 427(2):321-5
- Direct visualization of membrane leakage induce by the antibiotics peptides: maculatin, citropin and aurein. Ambroggio e.e., Separovic F., Bowie J.H., Fidelio G.D. and Bagatolli L.A. 2005. Biophys. J.
- Ligand-Indiced thermostability in proteins: thermodynamics análisis os ANS-albumin interaction. Celej MS, Hyrax SA, And Freire, Bianconi ML and Fidelio GD. Biochem. Biophys. Acta. 2005, 1750, 122-133
- Surface Behavior and Lipid Interaction of Alzheimer {beta}-Amyloid Peptide 1-42: a Membrane-disrupting Peptide. Ambroggio EE, Kim DH, Separovic F, Barrow CJ, Barnham KJ, Bagatolli LA. and Fidelio GD. Biophys J. 2005, 88: 2706-2713
(See more publications-CONICET)
Current Grants
- "Biophysical studies of structure and stability of lipolytic amphipathic proteins and bioactive peptides in their interaction with biomembranes". PICT-2011. FONCYT. granted. Execution start late 2012 TOTAL AMOUNT: $ 291.200 (3rd year running).
- "Biophysical studies of structure and stability of lipolytic amphipathic proteins and bioactive peptides in their interaction with biomembranes". PIP-CONICET 2012-2014.
- "Biophysical studies of structure and stability of lipolytic amphipathic proteins and bioactive peptides in their interaction with biomembranes". SECYT-UNC 2014-2015. RODE: $ 24.000
Brief CV
Academic Formation
I have received my first degree of Biochemist in 1979 at the Faculty of Chemistry Science, National University of Cordoba (the oldest University in Argentina); and my Ph.D. in Biochemistry in 1984 at the same University. Since then, I was involved in teaching and research in the field of Biophysics. I have been at the London University from 1985 to 1987 as posdoct (as Wellcome Trust Fellow, England). In my return to Argentina in 1987, I joined the Scientific Research Career of CONICET (from our National Research Council). Currently, I am in the category of Superior, the ultimate step of the scientific ladder in our Research Council. I am also Full Professor at Cordoba University. I have supervised many graduate and Ph.D. students, posdocts and graduate students as well. I have published over 80 papers in peer reviewed journals. In the past, I have participated with a chair as Committee Member in the Scientific and Promotion Council of our Province of Córdoba. I am also frequently acting as evaluator from our National Research Council (CONICET) and National Agency of Promotion of Science and Technology in Argentina (ANPCYT). I have also been abroad as visiting professor in Chile and Spain. Also, I am the past-president of the Argentinean Biophysical Society.
Teaching Background
- Leader teaching professor of the undergraduate Biophysical Chemistry course since Apr 1999.
- Coordinator and teaching participant of several posgraduate courses since 1995 in the membrane and protein biophysic field.
Institutional Management Background
- Director of CIQUIBIC, I. Cs. Chemical-UNC (Present since November 2015)
- Director of Department of Biological Chemistry, I. Cs. Chemical-UNC (Oct 2014-Nov 2015)
- Vice-Chancellor of National University of Cordoba, UNC. (Apr 2007-May 2009)
- Dean of Faculty of Chemical Science, UNC. (April 1999-April 2007)
- Secretary of Science and Technology, Faculty of Chemical Science, UNC. (Apr 1995- Apr 1999).
Directed Ph.D. Theses
- Dr. Luis Bagatolli (1995). “Lipid-lipid Interaction in glycosphingolipids containing interfaces. Applications of frequency domain fluorescence technique on different biological systems””. School of Chemistry, UNC. (Advisor)
- Dr. Jose Julian Daniele. (1997). “Structure-function and regulation of phospholipase A2”. School of Chemistry, UNC. (Advisor)
- Dr. Martin Gonzalez. (1998). “Effect of igands on thermal stability of proteins.”School of Chemistry, UNC. (Advisor)
- Dr. Ricardo Madoery. (1999). “Activity of immobilized Phosphilpase A2 enzymes””. School of Chemistry, UNC. (Advisor)
- Dr. Cristina Amulphi. (1999) “Hidration studies of gangliósidos by using 2H-NMR”. FAMAF, Faculty of Mathematics, Astronomy and Physics, UNC. (Co-Advisor)
- Dr. Michelina Pincheli. (1999)” Topology of lipid aggregates by using 31P-NMR”. FAMAF, Faculty of Mathematics, Astronomy and Physics, UNC. (Co-Advisor)
- Dr. Maria Soledad Celej.(2004). “Thermal stability of proteins induced by ligands and interfaces. Caracterization of conformational substates in proteins””. School of Chemistry, UNC. (Advisor).
- Dr. Ernesto E. Ambroggio.(2007). “Biophysical characterization of different amphiphatic peptides in their interaction with lipid interfaces”. School of Chemistry, UNC. (Advisor).
- Dr. Marta Inés Burgos. (2010). “Thermal stabilities in proteins: Effect of protein-ligand and protein-protein interaction”. School of Chemistry, UNC. (Advisor).
- Dr. Pablo Yunis Quartino. (2014) “Sequence and structure of phospholipase A2 from Bothrops diporus (yarará chica): relationship between kinetics with surface properties” School of Chemistry, UNC. (Advisor)
- Dr. Elisa Mariani. (2015) “Catalytic properties of soy bean (glycine max) phospholipase A2” School of Chemistry, UNC. (Co-Advisor)