CIQUIBIC-CONICET-UNC


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Integrantes

DURAND, Sandra

Investigadora Adjunta del CONICET / Profesora Asistente UNC
Teléfono: +54 351 5353855 interno 3442
E-mail: edithsandradurand@gmail.com

Tema de Investigación

Regulación de la función de la proteína básica de mielina por calmodulina y modificaciones post-traduccionales

La proteína básica de mielina (MBP) es esencial para la formación de las vainas de mielina en el sistema nervioso central. La interacción de MBP con diferentes componentes celulares es regulada por calmodulina (CaM). Además, esta interacción es influenciada por diversas modificaciones post-traduccionales (MPT) que sufre MBP. Una de las MPT es la deiminación o citrunilación que se correlaciona con el desarrollo temprano, el proceso de desmielinización y la severidad de la enfermedad en el caso de la esclerosis múltiple (MS). Con el fin de contribuir al conocimiento de la regulación de MBP por CaM estudiamos la interacción entre las distintas isoformas de la familia de MBP clásica y CaM y, además, cómo la deiminación/citrulinación de MBP modifica o no esta interacción. Nuestros hallazgos nos permiten afirmar que la actividad biológica de cada isoforma de MBP es regulada de manera diferencial por CaM y/o por modificaciones post-traduccionales. Por lo tanto, el estudio de esta regulación podría ser importante para comprender la función que cumplen estas isoformas en la diferenciación de los oligodendrocitos, la mielinización en condiciones normales y/o patológicas como en la MS, etc., y como la deimidación influye en estos eventos.

Publicaciones Seleccionadas

(Ver mas publicaciones-CONICET)

Colaboraciones

Breve Currículum Vitae

Formación Académica

 

  • Estudios Universitarios: Universidad Nacional de Córdoba, Facultad de Ciencias Químicas. Fecha de egreso: 8-10-1991. Título obtenido: Bioquímica.
  • Tesis doctoral: Universidad Nacional de Córdoba, Fac. de Cs. Qcas, Dpto. de Bqca. Clínica. Inscripta en la Carrera Doctoral el 23 de mayo de 1997 y admitida el 13 de agosto de 1997. Fecha de egreso: 11-08-2003. Título obtenido: Doctora en Ciencias Químicas. Título de la Tesis: “Identificación de genes diferencialmente expresados en trofoblasto normal y patológico. Caracterización de un nuevo transcripto que codifica para una proteína con dominio de unión a lípidos”.
  • Docente-Investigadora categoría III del Programa de Incentivos del Ministerio de Cultura y Educación – Secretaría de Políticas Universitarias desde el año 2005 hasta el presente. Categoría V: 1994-1999; Categoría IV: 2000-2004
  • Investigadora Asistente de la Carrera del Investigador Científico y Tecnológico (CICYT) desde el 01-07-2005 hasta el 31-05-2013. Disciplina: Bioquímica. Concurso 2004. Resolución N°: 2037/04. Resolución de designación N°: 820/05.
  • Investigadora Adjunta de la Carrera del Investigador Científico y Tecnológico (CICYT) desde el 01-06-2013 hasta el presente. Resolución N°: 1904.
  • Evaluadora del Organismo Argentino de Acreditación (OAA). Fecha de designación: 31-05-2011. Calificación y evaluación de desempeño y competencia de evaluadores realizada por el Comité de Evaluación el 09-10-2012: actuación satisfactoria.

Antecedentes en Investigación

 

Publicaciones

 

  • Leucemia Mieloide Crónica: relación entre el patrón molecular bcr-abl y el fenotipo celular de la crisis blástica. Durand S., Rodriguez C., Bocco J. y Núñez B. Presencia Bioquímica de la Asociación de Bioquímicos de Córdoba N° 178, volumen 15: 11-18, marzo de 1997.
  • Estudios clínicos y diagnóstico molecular de Distrofia Miotónica. Durand S., Slavin D., Molina de Levstein I. y Bocco J.L. Anales de la Fundación Alberto J. Roemmers, volumen XII: 49-56, 1999.
  • Identification of Trypanosoma cruzi zymodemes by kinetoplast DNA probes. Montamat E.E., Durand S., Bocco J.L., De Luca d’Oro G.M. and Blanco A. Journal of Eucaryotic Microbiology 46: 155-159, 1999.
  • Molecular mechanisms implicated in galectin-1-induced apoptosis: activation of the AP-1 transcription factor and downregulation of Bcl-2. Rabinovich G.A., Alonso C.R., Sotomayor C.E., Durand S., Bocco J.L. and Riera C.M. Cell Death and Differentiation 7: 747-753, 2000.
  • Altered mitochondrial gene expression in human gestational trophoblastic diseases. Durand S., Dumur C., Flury A., Abadie P., Patrito L., Podhajcer O. and Genti-Raimondi S. Placenta 22: 220-226, 2001.
  • A new death domain associated with gestational trophoblastic diseases induces apoptosis in distinct cell types. Dumur C.I., Almenara J.A., Durand S., Flury A., Koritschoner N.P. and Patrito L.C. International Journal of Oncology 19: 1161-1167, 2001.
  • Identification of multiple differentially expressed messenger RNAs in normal and pathological trophoblast. Durand S., Abadie P., Angeletti S. and Genti-Raimondi S. Placenta 24: 209-218, 2003.
  • GTT1/StarD7, a novel phosphatidylcholine transfer protein-like highly expressed in gestational trophoblastic tumour: cloning and characterization. Durand S., Angeletti S. and Genti-Raimondi S. Placenta 25: 37-44, 2004.
  • Evaluation of stromal metalloproteinases and vascular endothelial growth factors in a spontaneous metastasis model. Donadio A.C., Durand S., Remedi M.M., Frede S., Ceschin D.G., Genti-Raimondi S. and Chiabrando G.A. Experimental and Molecular Pathology 79: 259-264, 2005.
  • The arginylation-dependent association of calreticulin with stress granules is regulated by calcium. Carpio M.A., López Sambrooks C., Durand E.S. and Hallak M.E. Biochemical Journal 429: 63-72, 2010.
  • Galectin-1 confers immune privilege to human trophoblast: implications in recurrent fetal loss. Ramhorst R.E., Giribaldi L., Fraccaroli L., Toscano M.A., Stupirski J.C., Romero M.D., Durand E.S., Rubinstein N., Blaschitz A., Sedlmayr P, Genti-Raimondi S., Fainboim L. and Rabinovich G.A. Glycobiology 22: 1374-1386, 2012.
  • Calreticulin-dimerization induced by post-translational arginylation is critical for stress granules scaffolding. Carpio M.A., Decca M.B., Lopez Sambrooks C., Durand E.S., Montich G.G. and Hallak M.E. The International Journal of Biochemistry and Cell Biology 45: 1223-1235, 2013.

Antecedentes Docentes

 

  • Cargos de Auxiliar Docente del Dpto. de Bqca. Clínica, Fac. de Cs. Qcas., Universidad Nacional de Córdoba. Asignatura: Química Biológica Analítica. Desde el 01-12-1992 hasta el 31-03-2003.
  • Cargo de Auxiliar Docente del Dpto. de Qca. Biológica interino, Fac. de Cs. Qcas., Universidad Nacional de Córdoba. Asignaturas: Biología Celular y Molecular, Laboratorio IV. Desde el 01-07-2004 hasta el 31-07-2004.
  • Cargo de Profesor Asistente (DS) del Dpto. de Qca. Biológica por concurso, Fac. de Cs. Qcas., Universidad Nacional de Córdoba. Función Docente: Asignaturas del Departamento. Función de Investigación: Biología Celular y Molecular. Desde el 1° de abril de 2008 hasta el 31-03-2013. Resoluciones N°: 719 y 823. Desde el 1° de abril de 2013 hasta el 31-03-2015. Resoluciones N°: 819 y 1032. Desde el 1° de abril de 2015 hasta el 31-12-2015. Resolución N°: 360.

 

Research Topic

Regulation of the myelin basic protein function by calmoduline and post-tranlational modifications



The myelin basic protein (MBP) is essential for the formation of the myelin sheath in the central nervous system. MBP’s interaction with different cellular components is regulated by calmodulin (CaM). In addition, this interaction is influenced by various post-translational modifications (MPT) undergone by MBP. One of the MPT is the deimination or citrullination which correlates with the early development, demyelination and severity of disease in the case of multiple sclerosis (MS).

In order to contribute to the knowledge of the MBP regulation by CaM we study the interaction between the different isoforms of classical MBP family and CaM and also how the deimination/citrullination of MBP modified or not this interaction.

Our findings allow us to affirm that the biological activity of each isoform of MBP is differentially regulated by CaM and/or MPT. Therefore, the study of this regulation may be important in understanding the role of these isoforms in the differentiation of oligodendrocytes, the myelination in normal and pathological conditions as in MS, etc., and as the deimination influences those events.

Publications

Collaborators

  • Bosc Christophe y Annie Andrieux – Centre de Recherche Inserm U 836-UJF-CEA-CHU, Grenoble. Institut des Neurosciences, Université Joseph Fourier. Faculté de Médecine, Domaine de la Merci, F-38700 La Tronche, France

Brief CV

Academic Formation

  • Elementary and Middle School: Manuel Belgrano School of Villa Huidobro, General Roca Department, Córdoba Province. Year of graduation: 1980.
  • High School: Private Institute Incorporated to formal education: “The Sacred Hearts”” of Villa Huidobro, General Roca Department, Córdoba Province. Year of graduation: 1985. Degree: Bachelor in Teaching Guidance.
  • University: National University of Córdoba, Faculty of Chemistry. Graduation date: 10/08/1991. Degree: Biochemistry.
  • Oncohematology Laboratory Training: National University of Córdoba, Faculty of Medicine, National Hospital of Clinical, Oncohematology Department, from 01/06/1991 to 06/11/1996.
  • PhD: National University of Córdoba, Faculty of Chemistry, Clinical Biochemistry Department. Registered in the PhD Career on 23 May, 1997 and accepted on 13 August, 1997. Graduation date: 11/08/2003. Degree: PhD in Chemistry. PhD title: “Identification of genes differentially expressed in normal and pathological trophoblast. Characterization of a novel transcript encoding a protein with lipid binding domain”.
  • PosDoc: National University of Córdoba, Faculty of Chemistry, Biological Chemistry Department, from 01/04/2004 to 30/06/2005. PosDoc title: “Control study of the phospholipid biosynthesis rate in tumor cells activated by c-Fos through a nongenomic mechanism with therapeutic purposes”.
  • Assistant Research of the Career of Scientific and Technological Research (CICYT-CONICET) from 01/07/2005 to 31/05/2013. Discipline: Biochemistry.
  • Associate Research of the Career of Scientific and Technological Research (CICYT-CONICET) since 01/06/2013 to date.
  • Positions of Auxiliary Professor at the Clinical Biochemistry Department, Faculty of Chemistry, National University of Córdoba, from 01/12/1992 to 31/03/2004.
  • Positions of Auxiliary Professor at the Biological Chemistry Department, Faculty of Chemistry, National University of Córdoba, from 01/07/2004 to 31/03/2008.
  • Position of Assistant Professor at the Biological Chemistry Department, Faculty of Chemistry, National University of Cordoba, since 01/04/2008 to date.
  • Teacher-Researcher from Incentive Program of the Ministry of Culture and Education – University Policies Secretariat; category V: 1994-1999, category IV: 2000-2004 and category III since 2005 to date.
  • Referee of the Argentine Accreditation Organism (OAA). Date appointed: 31/05/2011; in activity since then to date.

Research Background

Publications

  • Leucemia Mieloide Crónica: relación entre el patrón molecular bcr-abl y el fenotipo celular de la crisis blástica. Durand S., Rodriguez C., Bocco J. y Núñez B. Presencia Bioquímica de la Asociación de Bioquímicos de Córdoba N° 178, volumen 15: 11-18, marzo de 1997.
  • Estudios clínicos y diagnóstico molecular de Distrofia Miotónica. Durand S., Slavin D., Molina de Levstein I. y Bocco J.L. Anales de la Fundación Alberto J. Roemmers, volumen XII: 49-56, 1999.
  • Identification of Trypanosoma cruzi zymodemes by kinetoplast DNA probes. Montamat E.E., Durand S., Bocco J.L., De Luca d’Oro G.M. and Blanco A. Journal of Eucaryotic Microbiology 46: 155-159, 1999.
  • Molecular mechanisms implicated in galectin-1-induced apoptosis: activation of the AP-1 transcription factor and downregulation of Bcl-2. Rabinovich G.A., Alonso C.R., Sotomayor C.E., Durand S., Bocco J.L. and Riera C.M. Cell Death and Differentiation 7: 747-753, 2000.
  • Altered mitochondrial gene expression in human gestational trophoblastic diseases. Durand S., Dumur C., Flury A., Abadie P., Patrito L., Podhajcer O. and Genti-Raimondi S. Placenta 22: 220-226, 2001.
  • A new death domain associated with gestational trophoblastic diseases induces apoptosis in distinct cell types. Dumur C.I., Almenara J.A., Durand S., Flury A., Koritschoner N.P. and Patrito L.C. International Journal of Oncology 19: 1161-1167, 2001.
  • Identification of multiple differentially expressed messenger RNAs in normal and pathological trophoblast. Durand S., Abadie P., Angeletti S. and Genti-Raimondi S. Placenta 24: 209-218, 2003.
  • GTT1/StarD7, a novel phosphatidylcholine transfer protein-like highly expressed in gestational trophoblastic tumour: cloning and characterization. Durand S., Angeletti S. and Genti-Raimondi S. Placenta 25: 37-44, 2004.
  • Evaluation of stromal metalloproteinases and vascular endothelial growth factors in a spontaneous metastasis model. Donadio A.C., Durand S., Remedi M.M., Frede S., Ceschin D.G., Genti-Raimondi S. and Chiabrando G.A. Experimental and Molecular Pathology 79: 259-264, 2005.
  • The arginylation-dependent association of calreticulin with stress granules is regulated by calcium. Carpio M.A., López Sambrooks C., Durand E.S. and Hallak M.E. Biochemical Journal 429: 63-72, 2010.
  • Galectin-1 confers immune privilege to human trophoblast: implications in recurrent fetal loss. Ramhorst R.E., Giribaldi L., Fraccaroli L., Toscano M.A., Stupirski J.C., Romero M.D., Durand E.S., Rubinstein N., Blaschitz A., Sedlmayr P, Genti-Raimondi S., Fainboim L. and Rabinovich G.A. Glycobiology 22: 1374-1386, 2012.
  • Calreticulin-dimerization induced by post-translational arginylation is critical for stress granules scaffolding. Carpio M.A., Decca M.B., Lopez Sambrooks C., Durand E.S., Montich G.G. and Hallak M.E. The International Journal of Biochemistry and Cell Biology 45: 1223-1235, 2013.

Teaching Background

I did my degree in Biochemistry at the Faculty of Chemistry, National University of Córdoba (FCQ-UNC) (1986-1991). I worked in the laboratory of the Oncohematology Department, National Hospital of Clinical, Faculty of Medicine, UNC (1991-1996). Since 1992 I teach at the FCQ-UNC. I did my PhD at the Clinical Biochemistry Department, FCQ-UNC (1997-2003). I did my postdoc at the Biological Chemistry Department, FCQ-UNC (2004-2005). I started the Career of Scientific Research of the National Council of Scientific and Technical Research (CICYT-CONICET) in 2005, being actually Associate Research. In 2011 I started working for the Argentine Accreditation Organism (OAA). Throughout my professional (Biochemistry) and scientific (PhD in Chemistry) career I have specialized in molecular biology techniques for diagnosis and research respectively. In recent years I have added to my expertise cell biology techniques in order to analyze the effect of post-translational modifications in cell function.

Institutional Management Background

  • Member of the Commission on Practical Work by the Biological Chemistry Department, Faculty of Chemistry, National University of Córdoba, during the years 2004, 2005 and 2006.
  • Tutor of the Tutoring Institutional Program by the Biological Chemistry Department, Faculty of Chemistry, National University of Córdoba, from June 16, 2006.
  • Member of the Departmental Council for the Biological Chemistry Department, Faculty of Chemistry, National University of Córdoba, from 14 October 2010 until 13 October 2012.
  • Delegate of the Biological Chemistry Department for the organization of the 6th Conference of Graduate Faculty of Chemistry, National University of Córdoba, held during 5 and 6 December 2013.