CIQUIBIC-CONICET-UNC


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Staff

FANANI, M. Laura

Independent Researcher CONICET / Adjunct Professor UNC
Phone: +54 351 5353855 ext 3438
E-mail: lfanani@fcq.unc.edu.ar

Research topic

Our laboratory aims to study the regulation of drug interaction with lipid membranes and biophysical model bases that modulate the effects of: I) the selectivity of drug uptake by membranes with different physical characteristics, structural and rheological, II) action at the metabolic level and III) permeation through membranes and hydrophobic structures such as the stratum corneum. As a study we focused on alkyl esters of L-ascorbic acid (ASCN), potent antioxidants and amphiphilic drugs called self-organized nanostructures coageles. We also studied the amphiphilic drug miltefosine or hexadecylphosphocholine (HePC), currently it used clinically for the treatment of cutaneous leishmaniasis and. Currently we are studying the regulation of incorporation of drugs to membranes model and physicochemical properties important for “Transdermal penetration of drugs favored”. We also studied the effect of incorporating these agents on lipid metabolism enzymes belonging to both of its enzymatic activity and structural effect their products have on the host membrane.

Scholars Group members

  • Daniel Alejandro Peñalva (CONICET researcher Asistrente, INIBBIB, co-direction)

Scholars Group members

Selected Publications

Collaborations

current subsidies

  • Director of Research Projects FONCyT, PICT 2014_1627 Open Topics Type D. Theme: "Interaction of drugs with biological membranes amphiphilic model". Period 2015-2018 (total amount $ 240000).
  • Director of Research Projects Type A SECyT-Univ. Nacional de Córdoba entitled "amphiphilic DRUG INTERACTION WITH BIOLOGICAL MEMBRANE MODEL". Period 2016-2018 (total 30000$).

Brief Curriculum Vitae

  • Degree in Biochemistry, (1991- 1996), School of Chemistry, UNC. Average 9.03 (nine points three hundredths) (Cod. UNAC0189).
  • Doctor of Chemical Sciences, (1996 – 2001), School of Chemistry, UNC, with outstanding rating. The thesis work developed under the direction of Dr.. Bruno Maggio, title “supramolecular modulating the activity of sphingomyelinase in biointerfases”. DOI: 10.13140/RG.2.1.2157.4008

Background Research

Dr.. Fanani graduated from Lic. In Biochemistry 1996 in the FCQ-UNC and began his doctoral studies under the direction of Dr. Bruno Maggio under the theme "supramolecular Modulation sphingomyelinase activity in biointerfases" that ended in the 2001 and for which he received the Award Dr. Agustin Marenzzi, 2000/2001 awarded by the Faculty of Pharmacy and Biochemistry, Univ. Buenos Aires. On this subject also he gave a brief postdoctoral work. Between 2003-2005 He studied on "Lipid Modulation of the Diacylglycerol Kinase Activity of α - and γ -Isoforms" under the direction of Dr. Richard Epand en la McMaster Univ. (Canada). After his return, Inv income as. Assistant (2005) and then as Deputy (2008) CONICET to CIQUIBIC focusing their study on the interfacial restructuring suffering lipid membranes when subjected to the action of lipolytic enzymes. It is well internalized in the structural properties (domain formation), rheological and thermodynamic model lipid membranes (especially lipid monolayer), particularly those containing sphingolipid ceramide and sphingomyelin simple as. Currently she holds the position of Prof. Deputy and Inv. Independent CONICET in CIQUIBC focusing its research on the interaction of small amphiphiles with pharmacological interest with model lipid membranes and how it depends on the biophysical properties of both the acceptor membrane as drugs themselves. He has directed three theses of degree and currently runs two doctoral theses, He has published over forty papers in international journals of which eight are revisions and has received more than 720 Citations (índice h 16). It has an active participation in conferences and postgraduate courses. He has received numerous trainees in his laboratory primarily interested in viewing by Brewster angle microscopy lipid monolayers, This has led to collaborations with the University. National South, la Univ. Havana and the Basque Country. He has participated in the Red network Bio-Nano-Science in the Midwest PAE 22642 "Self-Organization of bio-nanostructures for transmitting information in neurobiology and molecular biological processes" between the 2006-2009 and the Network of Ibero-American Science and Technology for Development (CYTED) Toxins of interest to biomedicine, 2012-2015. He is an active member of the Soc. Argentina Biophysics (SAB) and Soc. Argentina Research in Biochemistry and Cell Biology (REVIEW).

Dr.. Fanani has developed teaching activities from 1997 (1997- 2011 and from Assistant Professor 2011 so far as Assistant Professor) in the subject "Biophysical Chemistry" at the Faculty of Chemistry, UNC. At 2018 He will be in charge of the Laboratory Course IV, compulsory for all races of our Faculty. It also assists in teaching "Biological Chemistry" issues related to enzymes and enzyme activity. Since 2006 He collaborates in teaching postgraduate course “Molecular Biophysics Biomembranes”, directed by Dr.. Bruno Maggio to 2014 and that in the 2016 He was in charge, in the Department of Biological Chemistry. To date he has directed two doctoral theses, one of which ended in the 2016 with summa cum laude and has overseen three theses grade. It has an active participation in contests courts and doctoral thesis.

  • Member of the following committees of the FCQ-UNC: Teaching Assistant (2002-2003); Monitoring Study Plan (2007-2009); implementing career in Chemistry Teaching (2009-2011 and 2015-current); Partial coordination, (2012 – 2016).

  • Council member Deptal. Biological Chemistry 2006-2008 and 2016- in progress.
  • Member of the organizing committee of the following scientific meetings: School binational Nano-Science 2008; XXXIX Annual Meeting of the SAB 2010; XLII Annual Meeting of the SAB 2013; 3Meeting ra NANO-Córdoba 2014.

  • Member of the Evaluation Commission of Projects and Programs SECyT-UNC; Chemistry area (April 2016- in progress)
  • Evaluation of scientific projects FONCyT, CONICET and Univ. from Buenos Aires;
  • Evaluation of scientific staff of CONICET;
  • Review of manuscripts for scientific journals.

Blog creation and maintenance (http://marialaurafanani.blogspot.com.ar/) where scientific and educational activities that are involved laboratory members from diffuses 2009-ongoing. At 2017 Project runs a joint where it is proposed to implement laboratory experiments in Natural Sciences in primary schools.

  • Lic. Gail. Tema de tesis doctoral: "Structural characterization of liposomes and their interaction with amphiphilic molecules of pharmacological interest", Fcq- UNC”, dec. 2012- dec. 2016. Foncyt developed scholarship and CONICET. UNC School of Chemistry (Res. 31/13). Qualification: outstanding. Currently Dr.. Giudice develops a post-doctoral work at the INFIQC.
  • Bioq. Yenisleidy of Zulueta Mercedes Diaz. Tema de tesis doctoral: "Interaction of drugs with biological membranes amphiphilic model", FCQ-UNC. Developed with CONICET Scholarship, nov. 2014- in progress..

Research Topic

The biophysical properties of lipid membranes and its interaction with soluble amphiphiles.

Our laboratory proposes to study the regulation of drug interaction with model lipid membranes and biophysical bases that modulate the effects of: I) the selectivity of drug uptake by membranes with different physical, structural and rheological characteristics; II) its action at the metabolic level and III) the drug permeation capacity through membranes or hydrophobic structures such as the stratum corneum. As an object of study, we concentrate on the alkyl esters of L-ascorbic acid (ASCN), potent antioxidant and amphiphilic drugs that are autorangized in nanostructures called coageles. On the other hand, we studied the amphiphilic drug miltefosine or hexadecylphosphocholine (HePC), currently used clinically for the treatment of cutaneous lymphoma and leishmaniasis. At this time, we are studing the regulation of the incorporation of drugs into model membranes and the physicochemical properties important for the “transdermal drug penetration” effect. We also studied the effect of the incorporation of these drugs on enzymes belonging to the lipid metabolism both on their enzymatic activity and on the structural effect that their products exert on the host membrane.

Researchers members of the group

  • Peñalva, Daniel Alejandro (Assistant Investigator of CONICET; INIBBIB, co-supervisor)

Fellows

Selected Publications

(More Publications-CONICET)

Collaborators

Dra. Natalia Wilke (Independent Resercher CIQUIBIC)

Dr. Bruno Maggio (Senior Researcher CIQUIBIC)

Dr. Ernesto Ambroggio (Associate Researcher CIQUIBIC)

Dra. Raquel Vico (Associate Researcher INFICQ)

Dr. Fernando Dupuy (Assistant Researcher INSIBIO)

Dr. Luciano Benedini (Univ. Nacional del Sur)

Dra. Marta Aveldaño (INIBBIB)

Mgt. Lohans Pedrera Puentes (Univ. de la Habana, Cuba)

Dr. Carlos Alvarez Valcarcel (Univ. de la Habana, Cuba)

Dr. Felix Goñi (Univ. Of the Basque Country)

Dr. Joao Neto Ruggiero (Univ. of Sao Paulo, Brazil)

Current Grants

  • Research Project SECyT-Univ. Nacional de Córdoba, entitled “Biophysical characterization of model membranes containing simple sphingolipids and their interaction with amphiphiles of pharmacological interest.” Period 2014-2016 ($ Total 19000).
  • Research Projects FONCyT, PICT 2014_1627 Open Topics, Type D. Topic: “Interaction of amphiphilic drugs with lipid membrane model.” Period 2015-2018 ($ 240,000).

Brief CV

Academic Formation
Degree in Biochemistry (1991 March 11, 1996), School of Chemical Sciences, Universidad Nacional de Córdoba. Average 9.03 (nine point three hundredths).

Doctor of Chemical Sciences, (September 1996 – October 17, 2001), School of Chemical Sciences, Universidad Nacional de Córdoba, with a rating of outstanding. The thesis work was developed under the direction of Dr. Bruno Maggio, entitled “Supramolecular Modulation of sphingomyelinase activity in biointerfases”.

Research Background
Dr. Fanani graduated from Biochemistry in 1996 at FCQ-UNC and began her doctoral studies under the direction of Dr. Bruno Maggio under the theme “Supramolecular modulation of the activity of sphingomyelinase in biointerphases”, which ended in 2001 and for which he received the Dr. Agustin Marenzzi Prize, 2000/2001 awarded by the Faculty of Pharmacy and Biochemistry, Univ. Buenos Aires. She also developed a brief postdoctoral work following this subject in Dr. May. During 2003-2005, she developed studied on “Lipid Modulation of the Diacylglycerol Kinase Activity of α – and γ -Isoforms” under the supervision of Dr. Richard Epand at McMaster University (Canada). After her return, she obtained a position as Assistant researched (2005) and then as Associate Researcher of CONICET (2008) at CIQUIBIC, focusing her studies on the interfacial restructuring suffering by lipid membranes when they are subjected to the action of lipolytic enzymes. In this way, she deepened in the study of the structural (domain formation), rheological and thermodynamic properties of model lipid membranes (mainly lipid monolayers), in particular those containing simple sphingolipids such as ceramide and sphingomyelin. Currently she holds the position of Associate Professor and Independent CONICET Researcher at CIQUIBC, focusing her research on the interaction of amphiphiles of pharmacological interest with model lipid membranes, and how such interaction depends on both the drugs properties and the biophysical properties of the target membrane She has published over forty papers in international scientific journals, of which eight are reviews and has received more than 720 citations (index h 16). Dr. Fanani has received numerous trainees in her laboratory primarily interested in visualizing lipid monolayers by Brewster angle microscopy, which has led to collaborations with the National Univ. South Univ. of Havana, the Univ. of Sao Paulo, Brazil and the Univ. of the Basque Country. She has participated in the Bio-Nano-Science network of the center of the country PAE 22642 “Self-organization of bio-nanostructures for transmitting information in neurobiology and molecular biological processes” 2006-2009 and the Ibero-American Network Science and Technology Program for Development (CYTED) “Toxins of interest to biomedicine” 2012-2015. She is an active member of the Soc. Argentina Biophysics (SAB) and Soc. Arg. Research in Biochemistry and Cell Biology (REVIEW).

Teaching Background
Dr. Fanani has developed teaching activities since 1997 (1997- 2011 as Teacher Assistant and from 2011 to date as Associated Professor) on the fields of Molecular Biophysics in the College of Chemical Sciences, UNC. Beginning in 2018, Dr. Fanani will be in charge of the course Laboratory IV, mandatory for all the careers of our School. She also collaborate in the teaching of topics in Biological Chemistry related to enzymes and enzymatic activity for undergraduate courses. Since 2006 she collaborate in the teaching of the Graduate course “Molecular Biophysics of Biomembranes” directed by Dr. Bruno Maggio in the Department of Biological Chemistry. Up to date she has supervised three theses of degree and currently runs the direction of two doctoral theses. She has an active participation in conferences and postgraduate thesis committees.

Directed Ph.D. Theses

  • Lic. Gail. PhD thesis Title: “Structural characterization of liposomes and their interaction with amphiphilic molecules of pharmacological interest”, FCQ-UNC “, dec. 2012- Dec. 2016. Developed with Foncyt and CONICET scholarship. School of Chemical Sciences UNC (Res. 31/13). Score: outstanding. Currently Dr. Giudice develops a postdoctoral work at INFIQC.
  • Bioq. Yenisleidy of the Mercedes Zulueta Diaz. PhD thesis Title: “Interaction of amphiphilic drugs with model biological membranes”, FCQ-UNC. Developed with CONICET Scholarship, nov. 2014 – ongoing