Assistant Researcher CONICET / UNC Assistant Professor
Director: Carlos Argaraña
Phone: +54 351 5353855 ext 3463
Study of DNA repair mechanisms involved in the maintenance of genomic stability.
DNA is continuously exposed to different agents that threaten the stability of its genetic information. Different molecular DNA repair mechanisms have evolved within cells. They maintain the amount of changes in the cell to a range compatible with its normal functioning. My laboratory’s focus is the study of DNA repair mechanisms with an emphasis on the physical laws that govern protein-DNA interaction. My past work was on the Mismatch Repair System (MMR) studying endonuclease activity of MutL from P. aeruginosa. These results helped understand the factors that regulates MutL endonuclease activity and its implications on the MMR. I currently focus on the repair of double stranded-breaks by Homologous Recombination (HR) in S. cerevisiae. A wide range of methodologies are employed in this line of study. They encompass traditional biochemical assays, to a cutting-edge DNA curtain methodology. The DNA curtains allow the observation of hundred of aligned DNA molecules by TIRF microscopy. We have developed an assay for the visualization of the ssDNA bound with the single strand binding protein RPA and the Rad51 recombinase. The generation in vitro of the ssDNA-RPA and ssDNA-Rad51 filaments allow the study of the HR process in real time and at a single-molecule level.
- Luisina De Tullio *, Elisa M. E. Correa*, Mariana A. Martina, Paul S. Velez, Carlos E. Argaraña, José L. Barra. (2013). *Both authors contributed equally. Analysis of DNA structure and sequence requirements for Pseudomonas aeruginosa MutL endonuclease activity. The Journal of Biochemistry. 154(6), 505-511.
- Luisina De Tullio, Mary L. Fanani, Bruno Maggio. (2013). Surface mixing of products and substrate of PLA2 in enzyme-free mixed monolayers reproduce enzyme-driven structural topography. BBA biomembranes. 1828, 2056–2063.
- Elisa M. E. Correa, Mariana A. Martina, Luisina De Tullio, Carlos E. Argaraña, José L. Barra. (2011). Some amino acids of the Pseudomonas aeruginosa MutL D(Q/M)It has(X)2E(X)4E conserved motif are essentials for the in vivo function of the protein but not for the in vitro endonuclease activity. DNA Repair. 10, 1106-1113.
- Luisina De Tullio, Bruno Maggio, M. Laura Fanani. (2008). Sphingomyelinase acts by an area-activated mechanism on the liquid-expanded phase of sphingomyelin monolayers. Journal of Lipid Research. 49, 2347-2355.
- Luisina De Tullio, Bruno Maggio, Steffen Hartel, Jorge Jara, M. Laura Fanani. (2007). The initial surface composition and topography modulate sphingomyelinase-driven sphingomyelin to ceramide conversion in lipid monolayers. Cell Biochemistry and Biophysics. 47, 169-177.
- CONICET. PIP 20130100360.
- Pew Charitable Trusts. 27354.
- 2005-"Biologist". Equivalent to Bachelor of Science in Biology. National University of Cordoba.
- 2010-Ph.D. in Chemical Science. National University of Cordoba. ” Research Background “- 2013 – present. Assistant Researcher. CONICET.
- 2015-present. Postdoctoral fellow. Pew. Columbia University.
- 2010 – 2013 – Postdoctoral fellow. CONICET. ” Teaching Background – 2008
- 2016-Junior Teaching Instructor. National University of Cordoba.
Institutional Management Background
- Council member for the Department of Biological Chemistry. College of Chemical Sciences. UNC. 2012 – 2014.
- Member of the commission of laboratory classes for the Department of Biological Chemistry. College of Chemical Sciences. UNC. 2012 – 2014.