CIQUIBIC-CONICET-UNC


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DANIOTTI, Jose Luis

Principal Researcher CONICET / Full Professor UNC
Phone: +54 351 5353855 ext 3447
E-mail: daniotti@fcq.unc.edu.ar

Research topic

Synthesis and intracellular transport of glycolipids and acylated proteins

In our laboratory we are interested in studying the cellular processes that control the synthesis, intracellular distribution and degradation of glycolipids, amphipathic molecules synthesized in the Golgi complex through coordinated action of glycosyltransferase and located mainly in plasma membrane. The main objectives include the study of the synthesis and exocytic pathways and endocytic of glycolipids in cells of epithelial origin polarized and unpolarized. In addition, binding antibodies and intracellular fate of studying anti-glycolipids. A detailed knowledge of these processes will surely help elucidate potential of antibodies the anti-glycolipids associated with certain peripheral neuropathies. Another line of laboratory research is oriented to the study of the mechanisms that regulate protein acylation process and the consequences of covalent modification of the subcellular distribution of the same. The main objectives of this area of ​​research include the study of the processes of palmitoylation-depalmitoilación of model proteins Ras and GAP-43, and its consequence on the association to membranes and intracellular transport of these proteins. In addition, the functional role of the Ras and GAP-43 protein is investigated in magazines endocytic pathway, with particular emphasis on recycling endosomes.

Synthesis and intracellular transport of glycolipids and acylated proteins

In our laboratory we are interested in studying the cellular processes involved in the synthesis, intracellular distribution and degradation of glycolipids. These anphipatic molecules are synthesized in the Golgi complex through the coordinated action of glycosyltransferases and are mainly localized in the outer leaflet of the plasma membrane. In particular, we are interested in the synthesis, and endocytic and exocytic transport of glycolipids both in polarized and nonpolarized epithelial cells. We also study the binding, internalization and intracellular fate of anti-glycolipid antibodies. To know in detail these processes might help to understand the potential effect of anti-glycolipid antibodies associated to peripheral neuropathies. In another research line we investigate the cellular mechanisms that control and regulate the process of protein acylation as well as the consequences of this covalent modification on the subcellular distribution of the acylated polypeptides. In particular, we are examining the palmitoylation-depalmitoylation cycle of model proteins such as Ras and GAP-43 and its impact on membrane association and intracellular transport. We are also analyzing the functional roles of Ras and GAP-43 proteins in compartments of the endocytic pathway, with particular emphasis on the recycling endosomes compartment.

Researchers Group members

Scholars Group members

Selected Publications

(More publications-CONICET)

Brief Curriculum Vitae

University degrees

  • Biochemical, awarded by the Faculty of Chemical Sciences, Universidad Nacional de Córdoba. August 1987.
  • Doctor in Chemistry, awarded by the Faculty of Chemical Sciences, Universidad Nacional de Córdoba. Director: Dr. H.J.F. Maccioni. Córdoba, March 1992. Qualification: Outstanding.

Honours

  • "Outstanding Young Person of the Year" Award, edition 1999 of the Stock Exchange of Cordoba.
  • "Bernardo Houssay SECyT of the Scientific and Technological Research 2003" Award for Young Investigator, Biochemistry. Awarded by the Ministry of Science, Technology and Production of the Nation nInnovación. 5/2003.
  • "Hermann Burmeister" Genetics Cell Biology Award in the area ". Awarded by the National Academy of Sciences, Córdoba. 2/2004.
  • Recognition of TWAS (Academy of Sciences for the Developing World) para participar en la First Regional Conference of Young Scientist: Promoting Life Sciences for Sustainable Development. Angra do Reis, Brazil. 9/2006.
  • "Houssay Award 2010 in chemicals, Biochemistry and Molecular Biology ". Ministry of Science, Technology and Productive Innovation of Argentina. 3/2011.
  • "Pio Leon Distinction" award. Municipality of Jesús María, Córdoba. 10/2011.
  • Distinction "Outstanding Cordoba in 2011". Candidate of the Year Contest Córdoba newspaper La Voz del Interior. Córdoba, Argentina. 12/ 2011.

Management Activity and Academic Performance in Organizations

  • Secretary of Science and Technology, School of Chemistry, Universidad Nacional de Córdoba. July 2002 to June 2003. Res. 511/2002.
  • Deputy Director of the Department of Biological Chemistry, Faculty of Chemistry, Universidad Nacional de Córdoba. October 2004 to October 2006.
  • Counselor to the faculty Attachments to the HCD of the Faculty of Chemistry, UNC. 2000-2002.
  • Cs Coordinator Advisory Commission. Chemical SECyT-UNC. Julio 2002 to June 2003.
  • Cs member Advisory Commission. Chemical SECyT-UNC. October 2003 to December 2005.
  • Cs member Advisory Commission. Agricultural SECyT-UNC. Dic. 2005-2008.
  • Member of the Commission Reg. Surveillance and Faculty of Cs. Chemical, UNC. 2008-2010.
  • Director for the Faculty and Associate to the HCD of the Faculty of Chemistry, Universidad Nacional de Córdoba. 2008-2010.
  • Co-coordinator of the Scientific Evaluation System and Technology Projects (SEPCyT), FONCyT, Cell area Biological Sciences Molecules. 2010-2012
  • Director of the Department of Biological Chemistry, Faculty of Chemistry, Universidad Nacional de Córdoba. October 2010-2012.
  • Member of the Departmental Council of the Department of Biological Chemistry, Faculty of Chemistry, Universidad Nacional de Córdoba. October 2012-2014; 2014-2016.
  • Member of the Advisory Committee of Biochemistry and Molecular Biology. CONICET. 2013-2014.
  • Member of the Advisory Commission of the National System for Microscopy. Ministry of Science, Technology and Technological Innovation of Argentina. 2008-keep going.

Scientific Societies

National

  • Active member of the Argentina Society of Biochemistry and Molecular Biology Research (REVIEW).
  • Active member of the Soc. Argentina Research in Neuroscience (SAN).
  • Active member of the Society of Biology of Cordoba (SBC).

International

  • Miembro de la International Brain Research Organization (IBRO).
  • Miembro de la American Society for Cell Biology (ASCB).
  • Member of the International Organization Glycoconjugate (UP).

Doctoral thesis directed

  • Dr. ADOLFO R. ZURITA. Theme: "Effect of ganglioside expression on signal transduction mediated by epidermal growth factor" Completing: 10 December 2002. School of Chemistry, Universidad Nacional de Córdoba. Qualification: Outstanding.
  • Dr. CRESPO MARIA PILAR. Theme: “Gangliósidos: intracellular transport and distribution in epithelial cell membranes ". Ending: 26 August 2005. School of Chemistry, Universidad Nacional de Córdoba. Qualification: Outstanding.
  • Dr. WILLIAM A. GOMEZ. Theme: "Mechanisms of subcellular localization of isoforms H- and K- Ras protein ". Ending: 12 December 2007. School of Chemistry, Universidad Nacional de Córdoba. Qualification: Outstanding.
  • Dr. Ramiro Iglesias-Bartolome. Theme: "Exocytic and endocytic transport of ganglioside". Ending: 1 December 2008. School of Chemistry, Universidad Nacional de Córdoba. Qualification: Outstanding.
  • Dr. ALEJANDRA TRENCHI. Theme: "Characterization of the mechanisms of intracellular distribution and association endomembranas the S-acylated GAP-43 protein". Ending: 22 February 2010. School of Chemistry, Universidad Nacional de Córdoba. Qualification: Outstanding.
  • Dr. TORRES VANINA DEMICHELIS. Theme: "Characterization of glycosyltransferase on the cell surface glycolipid". Ending: 30 April 2013. School of Chemistry, Universidad Nacional de Córdoba. Qualification: Outstanding.

Research Topic

Biochemical and functional studies of glycolipids and acylated proteins

In our laboratory we are interested in studying the cellular processes involved in the synthesis, intracellular distribution and degradation of glycolipids. These anphipatic molecules are mostly synthesized in the Golgi complex through the coordinated action of glycosyltransferases and are mainly localized in the outer leaflet of the plasma membrane. In particular, we are interested in the synthesis, and endocytic and exocytic transport of glycolipids both in polarized and nonpolarized epithelial cells. We also study the binding, internalization and intracellular fate of ligands to glycolipid, mainly antibodies. To know in detail these processes might help to understand the potential effect of anti-glycolipid antibodies associated to peripheral neuropathies. In addition, as neosynthesized gangliosides observed in oncogenic processes show antigen specificity, the characterization of antibodies to gangliosides has also important conceptual and translational implications for the development of innovative therapeutics targeting and future design of immunotherapies for cancer.

In another research line we investigate the cellular mechanisms that control and regulate the process of protein acylation as well as the consequences of this covalent modification on the subcellular distribution of the acylated polypeptides. In particular, we are examining the palmitoylation-depalmitoylation cycle of model proteins such as Ras and GAP-43 and its impact on membrane association and intracellular transport. We are also analyzing the functional roles of Ras and GAP-43 proteins in compartments of the endocytic pathway, with particular emphasis on the recycling endosomes compartment.

Selected publications

(More publications-CONICET)

Researchers members of the group

Fellows

Postdoc

PhD students

Current Grants

  • Mizutani Foundation For Glycoscience. Japan. 2016-2017.
  • National Agency for Scientific and Technological Promotion (ANPCYT-FONCYT). Argentina, PICT-2013-0456. 2014-2017.
  • Ministry of Science and Technology, Universidad Nacional de Córdoba, Córdoba, Argentina. 2016-2018.
  • National Agency for Scientific and Technological Promotion (ANPCYT-FONCYT). Argentina, PICT-2015-1316. 2016-2019.

Brief CV

Academic Formation

  • 1987, Biochemist, School of Chemistry, Universidad Nacional de Córdoba, Argentina.
  • 1992, Ph.D., Department of Biological Chemistry, School of Chemistry, Universidad Nacional de Córdoba, Argentina. Title: “Metabolism of gangliosides during the development of the retina”. Ph.D. Advisor: Prof. Dr. Hugo J.F. Maccioni.

Research Background

  • 1988-1989, Fellowship from the National University of Córdoba, Scientific Research Council.
  • 1989, Fellow from Council of Scientific and Technological Research Córdoba.
  • 1989-1992, Fellow from the Argentinian Research Council (CONICET).
  • 1992-1994, Post-Doctoral Fellow from the International Center for Genetic Engineering and Biotechnology (ICGEB) at the Faculty of Medicine, University of Chile, Santiago, Chile. Chairman: Dr. Jorge E. Allende.
  • 1993, Fogarty Fellow at the Lab. of Dr. Silvio Gutkind (NIDCR, NIH). 06-09/1993.
  • 2000, Visiting Scientist at the Laboratory of Dr. R. Proia (NIDDK, NIH). 07-11/2000
  • 1997-Present, Member of the Career Investigators of the Argentinean Research Council (CONICET).

Teaching Background

  • 2009-Present. Full Professor in Cellular and Molecular Biology, College of Chemical Sciences, National University of Córdoba, Córdoba, Argentina.

Institutional Management Background

  • Secretary of Science and Technology, School of Chemistry, Universidad Nacional de Córdoba. 2002-2003.
  • Deputy Director of the Department of Biological Chemistry, School of Chemistry, Universidad Nacional de Córdoba. 2004-2006.
  • Director of the Department of Biological Chemistry, School of Chemistry, Universidad Nacional de Córdoba. 2010-2012.
  • Vice Director of the Center for Research in Biological Chemistry Córdoba (CIQUIBIC, UNC-CONICET). 2015-2020.

Background on Science outreach and extension

  1. Development of a methodology for the diagnosis of cholera through the detection of cholera toxin in different biological samples together with Drs. Maccioni HJF, Omar F, Barra JL, Rodriguez P and Monferran C and with the support of the Secretariat of Science and Technology of the Province of Córdoba, Argentina. The procedure was patented and transferred for commercialization.
  2. Member of the project “Biotechnology for Hemoderivatives” (Biohem, UNC-CONICET). EMPRETECNO from the Argentinean Sectorial Fund (FONARSEC) – Program of Support to Technologically Based Companies (PAEBT, Sector Biosalud). Awarded by the ANPCyT by resolution 425/2011 dated November 24, 2011. Objective: Production of recombinant Factor IX used for the treatment of patients suffering from Hemophilia B.

Directed Ph.D. Theses

  1. Dr. ADOLFO ZURITA. 2002.
  2. Dr. CRESPO MARIA PILAR. 2005.
  3. Dr. WILLIAM A. GOMEZ. 2007.
  4. Dr. Ramiro Iglesias-Bartolome. 2008.
  5. Dr. ALEJANDRA TRENCHI. 2010.
  6. Dr. VANINA TORRES-DEMICHELIS. 2013.
  7. Dr. PILAR PEDRO. 2016.