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"CIQUIBIC Open Doors" 2019

Second lecture by Dr.. Kierbel (IIBIO-INTECH-CONICET)

Next Thursday 30 May at 11 hours, Integrators in the Auditorium of the Faculty of Chemical Sciences of the National University of Córdoba, then give the Lecture Series External Researchers "CIQUIBIC Open Doors".

The conference will be headed by Dr.. Arlinet Kierbel, Independent researcher at the CONICET Research Institute biotechnological (IIBIO-INTECH-CONICET) National University of San Martin.

interaction Pseudomonas aeruginosa with host cells: since the formation of structures multicellular to eferocitosis.

Pseudomonas aeruginosa (PA) It is an opportunistic pathogen that can cause acute infections in burned or injured and chronic infections in patients with cystic fibrosis. In the latter a determinant of chronicity is the formation of biofilms. In the initial stages of the formation of bacterial biofilms as multicellular structures, It takes place the transition from a state to a multicellular planktonic, sessile and adhered. This transition has been little studied, especially when it occurs on biotic surfaces.

We have seen that when PA interacts with the epithelium is found almost exclusively multicellular unions. At these sites the bacteria adheres as aggregates extruded apoptotic cells. The formation of these multicellular structures occur de novo in the order of minutes. Through complementation of mathematical models and studies vivo microscopy we characterize the kinetics of formation of aggregates and chemotaxis determined that plays a role in the same.

also we determined that the bacteria added on dead cells can be internalized by the surrounding epithelial cells (as well as by macrophages) through the mechanism eferocitosis, a physiological process by which the dead cells are phagocytosed and processed. Once inside the cells PA is eliminated in lysosomes. then we propose, eferocitosis that would be part of a defense mechanism. Our findings are relevant to the study of infections with PA in contexts where they represent a problem of gravity, such as cystic fibrosis or wounds and burns, all characterized by the presence of a high number of dead cells.

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